ProSci

NKD2 Antibody

Product Code:
 
PSI-56-250
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
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NKD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis in Jurkat cell line lysates (35ug/lane).
NKD2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

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PSI-56-250-400ul400ul£626.00
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Further Information

Additional Names:
Protein naked cuticle homolog 2, Naked-2, hNkd2, NKD2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.
Background References:
  • Hu, T., et al. J. Biol. Chem. 285(18):13561-13568(2010)
  • Ding, W., et al. Proc. Natl. Acad. Sci. U.S.A. 105(36):13433-13438(2008)
  • Li, C., et al. Mol. Biol. Cell 18(8):3081-3093(2007)
  • Adamowicz, M., et al. Genes Chromosomes Cancer 45(9):829-838(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NKD2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-393 amino acids from the C-terminal region of human NKD2.
NCBI Gene ID #:
85409
NCBI Official Name:
Protein naked cuticle homolog 2
NCBI Official Symbol:
NKD2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q969F2
Protein GI Number:
74716653
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q969F2
User NOte:
Optimal dilutions for each application to be determined by the researcher.