ProSci

ARPC1A Antibody

Product Code:
 
PSI-62-296
Product Group:
 
Primary Antibodies
Supplier:
 
ProSci
Host Type:
 
Rabbit
Antibody Isotype:
 
Rabbit Ig
Antibody Clonality:
 
Polyclonal
Regulatory Status:
 
RUO
Target Species:
 
Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
 
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of WiDr cells using ARPC1A Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with ARPC1A Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of WiDr cells using ARPC1A Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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PSI-62-296-400ul400ul£626.00
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Further Information

Additional Names:
Actin-related protein 2/3 complex subunit 1A, SOP2-like protein, ARPC1A, SOP2L
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
ARPC1A is one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages.
Background References:
  • Laurila,E., Genes Chromosomes Cancer 48 (4), 330-339 (2009)
  • Machesky,L.M., Biochem. J. 328 (PT 1), 105-112 (1997)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This ARPC1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 286-315 amino acids from the C-terminal region of human ARPC1A.
NCBI Gene ID #:
10552
NCBI Official Name:
Actin-related protein 2/3 complex subunit 1A
NCBI Official Symbol:
ARPC1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q92747
Protein GI Number:
88984001
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
Q92747
User NOte:
Optimal dilutions for each application to be determined by the researcher.