NEU2 Antibody

ProSci
Product Code: PSI-56-269
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-269-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 5
Western Blot at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane.
2 / 5
Western blot analysis in A549 cell line lysates (35ug/lane).
3 / 5
4 / 5
Confocal immunofluorescent analysis of NEU2 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
5 / 5
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + human fetal liver lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in A549 cell line lysates (35ug/lane).
Confocal immunofluorescent analysis of NEU2 Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Sialidase-2, Cytosolic sialidase, N-acetyl-alpha-neuraminidase 2, NEU2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
This gene belongs to a family of glycohydrolytic enzymes which remove sialic acid residues from glycoproteins and glycolipids. Expression studies in COS7 cells confirmed that this gene encodes a functional sialidase. Its cytosolic localization was demonstrated by cell fractionation experiments. [provided by RefSeq].
Background References:
  • Stoppani, E., et al. Cell Biol. Int. 33(9):1020-1025(2009)
  • Li, C.Y., et al. Cell Res. 17(4):357-362(2007)
  • Chavas, L.M., et al. J. Biol. Chem. 280(1):469-475(2005)
  • Seyrantepe, V., et al. J. Biol. Chem. 279(35):37021-37029(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NEU2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-50 amino acids from the N-terminal region of human NEU2.
NCBI Gene ID #:
4759
NCBI Official Name:
Sialidase-2
NCBI Official Symbol:
NEU2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
42 kDa
Protein Accession #:
Q9Y3R4
Protein GI Number:
229462907
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q9Y3R4
User NOte:
Optimal dilutions for each application to be determined by the researcher.