HSPA8 Antibody

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ProSci
Product Code: PSI-61-566
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-566-400ul400ul£662.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from A431,Hela,mouse NIH/3T3,H-4-II-E cell line (from left to right),using HSPA8 Antibody .AP2872a was diluted at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysates from A431,Hela,mouse NIH/3T3,H-4-II-E cell line (from left to right),using HSPA8 Antibody .AP2872a was diluted at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with HSPA8 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Heat shock cognate 71 kDa protein, Heat shock 70 kDa protein 8, Lipopolysaccharide-associated protein 1, LAP-1, LPS-associated protein 1, HSPA8, HSC70, HSP73, HSPA10
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
HSPA8 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. HSPA8 is a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. The protein also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell.
Background References:
  • Tsukahara F., Yoshioka T.Mol. Pharmacol. 58:1257-1263(2000)
  • Egerton M., Moritz R.L.Biochem. Biophys. Res. Commun. 224:666-674(1996)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Hamster, Mouse, Rat
Immunogen:
This HSPA8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the N-terminal region of human HSPA8.
NCBI Gene ID #:
3312
NCBI Official Name:
Heat shock cognate 71 kDa protein
NCBI Official Symbol:
HSPA8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
71 kDa
Protein Accession #:
P11142
Protein GI Number:
123648
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Research Area:
Signal Transduction
Swissprot #:
P11142
User NOte:
Optimal dilutions for each application to be determined by the researcher.