SRP72 Antibody

ProSci
Product Code: PSI-62-535
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-535-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 4
Flow cytometric analysis of hela cells using SRP72 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunofluorescence analysis of SRP72 Antibody with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Western Blot at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: K562 whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of hela cells using SRP72 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Immunofluorescence analysis of SRP72 Antibody with hela cells . 0.025 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Further Information

Additional Names:
Signal recognition particle subunit SRP72, SRP72, Signal recognition particle 72 kDa protein, SRP72
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50

For IHC-P starting dilution is: 1:50~100

For IF starting dilution is: 1:10~50
Background:
Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. It binds the 7S RNA only in presence of SRP68. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.
Background References:
  • Utz,P.J., et.al., J. Biol. Chem. 273 (52), 35362-35370 (1998)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SRP72 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 119-148 amino acids from the Central region of human SRP72.
NCBI Gene ID #:
6731
NCBI Official Name:
Signal recognition particle subunit SRP72
NCBI Official Symbol:
SRP72
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
O76094
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
O76094
User NOte:
Optimal dilutions for each application to be determined by the researcher.