EXT2 Antibody

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ProSci
Product Code: PSI-63-927
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-927-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from T47D cell line,using EXT2 Antibody at 1:1000 at each lane
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Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Western blot analysis of lysate from T47D cell line,using EXT2 Antibody at 1:1000 at each lane
Formalin-fixed and paraffin-embedded human brain tissue reacted with EXT2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometry analysis of Hela cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Documents

Further Information

Additional Names:
Exostosin-2, Glucuronosyl-N-acetylglucosaminyl-proteoglycan/N-acetylglucosaminyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase, Multiple exostoses protein 2, Putative tumor suppressor protein EXT2, EXT2
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50

For IF starting dilution is: 1:10~50

For FACS starting dilution is: 1:10~50
Background:
EXT2 encodes one of two glycosyltransferases involved in the chain elongation step of heparan sulfate biosynthesis.
Background References:
  • Zhao, J., et al. Diabetes 59(3):751-755(2010)
  • Li, Y., et al. Genet Test Mol Biomarkers 13(6):825-830(2009)
  • Yerges, L.M., et al. J. Bone Miner. Res. 24(12):2039-2049(2009)
  • Heinritz, W., et al. Ann. Hum. Genet. 73 (PT 3), 283-291 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This EXT2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-209 amino acids from the Central region of human EXT2.
NCBI Gene ID #:
2132
NCBI Official Name:
Exostosin-2
NCBI Official Symbol:
EXT2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
82 kDa
Protein Accession #:
Q93063
Protein GI Number:
3023739
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Research Area:
Signal Transduction
Swissprot #:
Q93063
User NOte:
Optimal dilutions for each application to be determined by the researcher.