HIST2H2BF Antibody

ProSci
Product Code: PSI-58-399
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-399-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

Further Information

Additional Names:
Histone H2B type 2-F, HIST2H2BF
Application Note:
For WB starting dilution is: 1:1000
Background:
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Background References:
  • Kim, S.C., et al. Mol. Cell 23(4):607-618(2006)
  • Pavri, R., et al. Cell 125(4):703-717(2006)
  • Bonenfant, D., et al. Mol. Cell Proteomics 5(3):541-552(2006)
  • Zhu, B., et al. Mol. Cell 20(4):601-611(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Bovine, Pr
Immunogen:
This HIST2H2BF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 14-42 amino acids from the N-terminal region of human HIST2H2BF.
NCBI Gene ID #:
440689
NCBI Official Name:
Histone H2B type 2-F
NCBI Official Symbol:
HIST2H2BF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
Q5QNW6
Protein GI Number:
74743113
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q5QNW6
User NOte:
Optimal dilutions for each application to be determined by the researcher.