DNMT1 Antibody
| Code | Size | Price |
|---|
| PSI-55-132-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA (cytosine-5)-methyltransferase 1, Dnmt1, CXXC-type zinc finger protein 9, DNA methyltransferase HsaI, DNA MTase HsaI, MHsaI, MCMT, DNMT1, AIM, CXXC9, DNMT
Application Note:
For WB starting dilution is: 1:1000
Background:
Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Dnmt1 co-purifies with the retinoblastoma (Rb) tumour suppressor gene product, E2F1, and HDAC1. Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2Fbinding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
Background References:
- Peterson, E.J., et al., Cancer Res. 63(20):6579-6582 (2003).
- Leu, Y.W., et al., Cancer Res. 63(19):6110-6115 (2003).
- Saito, Y., et al., Int. J. Cancer 105(4):527-532 (2003).
- Siedlecki, P., et al., Biochem. Biophys. Res. Commun. 306(2):558-563 (2003).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This DNMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1083-1112 amino acids from human DNMT1.
NCBI Gene ID #:
1786
NCBI Official Name:
DNA (cytosine-5)-methyltransferase 1
NCBI Official Symbol:
DNMT1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
183 kDa
Protein Accession #:
P26358
Protein GI Number:
12231019
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P26358
User NOte:
Optimal dilutions for each application to be determined by the researcher.