HIST1H2AG Antibody

ProSci
Product Code: PSI-59-983
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-983-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Western blot analysis in MCF-7 cell line lysates (35ug/lane).

Further Information

Additional Names:
Histone H2A type 1, H2A1, Histone H2A/p, HIST1H2AG, H2AFP
Application Note:
For WB starting dilution is: 1:1000
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2A family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the histone microcluster on chromosome 6p21.33. [provided by RefSeq].
Background References:
  • Shi, J., et al. Nature 460(7256):753-757(2009)
  • Nicassio, F., et al. Curr. Biol. 17(22):1972-1977(2007)
  • Lusic, M., et al. EMBO J. 22(24):6550-6561(2003)
  • Marzluff, W.F., et al. Genomics 80(5):487-498(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Rat, Bovine, Mouse
Immunogen:
This HIST1H2AG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 102-130 amino acids from the C-terminal region of human HIST1H2AG.
NCBI Gene ID #:
8329, 8330, 8332, 8336, 8969
NCBI Official Name:
Histone H2A type 1
NCBI Official Symbol:
HIST1H2AG
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
14 kDa
Protein Accession #:
P0C0S8
Protein GI Number:
83288406
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P0C0S8
User NOte:
Optimal dilutions for each application to be determined by the researcher.