Leo1-S151 Antibody

ProSci
Product Code: PSI-60-715
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-715-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution + HL-60 whole cell lysate Lysates/proteins at 20 ug per lane.

Further Information

Additional Names:
RNA polymerase-associated protein LEO1, Replicative senescence down-regulated leo1-like protein, LEO1, RDL
Application Note:
For WB starting dilution is: 1:1000
Background:
LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).
Background References:
  • Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Beausoleil, S.A., et al. Nat. Biotechnol. 24(10):1285-1292(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Leo1-S151 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 129-158 amino acids from the N-terminal region of human Leo1-S151.
NCBI Gene ID #:
123169
NCBI Official Name:
RNA polymerase-associated protein LEO1
NCBI Official Symbol:
LEO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
75 kDa
Protein Accession #:
Q8WVC0
Protein GI Number:
74751545
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8WVC0
User NOte:
Optimal dilutions for each application to be determined by the researcher.