SETD1A Antibody

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ProSci
Product Code: PSI-60-677
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-677-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

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Western blot analysis in 293 cell line lysates (35ug/lane).
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Western blot analysis in 293 cell line lysates (35ug/lane).

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Further Information

Additional Names:
Histone-lysine N-methyltransferase SETD1A, Lysine N-methyltransferase 2F, SET domain-containing protein 1A, hSET1A, Set1/Ash2 histone methyltransferase complex subunit SET1, SETD1A
Application Note:
For WB starting dilution is: 1:1000
Background:
SET1A is a component of a histone methyltransferase (HMT) complex that produces mono-, di-, and trimethylated histone H3 at Lys4. The complex is the analog of the S. cerevisiae Set1/COMPASS complex (Lee and Skalnik, 2005 [PubMed 16253997]). Also see SET1B (MIM 611055).
Background References:
  • Wu, M., et al. Mol. Cell. Biol. 28(24):7337-7344(2008)
  • Nguyen, P., et al. Mol. Cell. Biol. 28(21):6720-6729(2008)
  • Ansari, K.I., et al. Biochim. Biophys. Acta 1779(1):66-73(2008)
  • Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SETD1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 927-956 amino acids from the Central region of human SETD1A.
NCBI Gene ID #:
9739
NCBI Official Name:
Histone-lysine N-methyltransferase SETD1A
NCBI Official Symbol:
SETD1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
186 kDa
Protein Accession #:
O15047
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O15047
User NOte:
Optimal dilutions for each application to be determined by the researcher.