MBD4 Antibody
| Code | Size | Price |
|---|
| PSI-60-515-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Methyl-CpG-binding domain protein 4, 322-, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, MBD4, MED1
Application Note:
For WB starting dilution is: 1:1000
Background:
DNA methylation is the major modification of eukaryotic
genomes and plays an essential role in mammalian development. Human
proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of
nuclear proteins related by the presence in each of a methyl-CpG
binding domain (MBD). Each of these proteins, with the exception of
MBD3, is capable of binding specifically to methylated DNA. MBD4
may function to mediate the biological consequences of the
methylation signal. In addition, MBD4 has protein sequence
similarity to bacterial DNA repair enzymes and thus may have some
function in DNA repair. Further, MBD4 gene mutations are detected
in tumors with primary microsatellite-instability (MSI), a form of
genomic instability associated with defective DNA mismatch repair,
and MBD4 gene meets 4 of 5 criteria of a bona fide MIS target gene.
Background References:
- Arora, M., et al. Leukemia 24(8):1470-1475(2010)
- Thyagarajan, B., et al. Biol. Blood Marrow Transplant. 16(8):1084-1089(2010)
- Ho-Pun-Cheung, A., et al. Pharmacogenomics J. (2010) In press :
- Briggs, F.B., et al. Am. J. Epidemiol. 172(2):217-224(2010)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This MBD4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 323-351 amino acids from the Central region of human MBD4.
NCBI Gene ID #:
8930
NCBI Official Name:
Methyl-CpG-binding domain protein 4
NCBI Official Symbol:
MBD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
66 kDa
Protein Accession #:
O95243
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O95243
User NOte:
Optimal dilutions for each application to be determined by the researcher.