PRMT5 Antibody

ProSci
Product Code: PSI-55-030
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-030-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti- in HL60 cell line lysates (35ug/lane).

Western blot analysis of anti- in HL60 cell line lysates (35ug/lane).

Further Information

Additional Names:
Protein arginine N-methyltransferase 5, 211-, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally processed, PRMT5, HRMT1L5, IBP72, JBP1, SKB1
Application Note:
For WB starting dilution is: 1:1000
Background:
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
Background References:
  • Pal, S., et al., Mol. Cell. Biol. 23(21):7475-7487 (2003).
  • Rho, J., et al., J. Biol. Chem. 276(14):11393-11401 (2001).
  • Pollack, B.P., et al., J. Biol. Chem. 274(44):31531-31542 (1999).
  • Gilbreth, M., et al., Proc. Natl. Acad. Sci. U.S.A. 95(25):14781-14786 (1998).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Xenopus
Immunogen:
This PRMT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-499 amino acids from the C-terminal region of human PRMT5.
NCBI Gene ID #:
10419
NCBI Official Name:
Protein arginine N-methyltransferase 5
NCBI Official Symbol:
PRMT5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
73 kDa
Protein Accession #:
O14744
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
O14744
User NOte:
Optimal dilutions for each application to be determined by the researcher.