SIGLEC10 Antibody
| Code | Size | Price |
|---|
| PSI-58-386-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Sialic acid-binding Ig-like lectin 10, Siglec-10, Siglec-like protein 2, SIGLEC10, SLG2
Application Note:
For WB starting dilution is: 1:1000
Background:
SIGLEC10 is a putative adhesion molecule that mediates sialic-acid dependent binding to cells. It preferentially binds to alpha2,3- or 2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. SIGLEC10 interacts with PTPN6/SHP-1 upon phosphorylation. The protein is expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform 5 is found to be the most abundant isoform, found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells. SIGLEC10 contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in downmodulation of cellular responses. The phosphorylated ITIM motif binds to the SH2 domain of PTPN6/SHP-1. Phosphorylation of Tyr-667 is involved in binding to PTPN6. The SIGLEC10 gene belongs to the immunoglobulin superfamily.
Background References:
- Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
- Kitzig, F., et al., Biochem. Biophys. Res. Commun. 296(2):355-362 (2002).
- Li, N., et al., J. Biol. Chem. 276(30):28106-28112 (2001).
- Yousef, G.M., et al., Biochem. Biophys. Res. Commun. 284(4):900-910 (2001).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SIGLEC10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 656-687 amino acids from the C-terminal region of human SIGLEC10.
NCBI Gene ID #:
89790
NCBI Official Name:
Sialic acid-binding Ig-like lectin 10
NCBI Official Symbol:
SIGLEC10
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
77 kDa
Protein Accession #:
Q96LC7
Protein GI Number:
118572721
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96LC7
User NOte:
Optimal dilutions for each application to be determined by the researcher.