HEYL Antibody

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ProSci
Product Code: PSI-58-862
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-862-400ul400ul£626.00
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Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
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Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.
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Western blot analysis in MDA-MB231 cell line lysates (35ug/lane).
Fluorescent confocal image of Hela cell stained with HEYL Antibody (N-term).HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HEYL primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HEYL immunoreactivity is localized to Mitochondria significantly.

Documents

Further Information

Additional Names:
Hairy/enhancer-of-split related with YRPW motif-like protein, hHeyL, Class B basic helix-loop-helix protein 33, bHLHb33, Hairy-related transcription factor 3, HRT-3, hHRT3, HEYL, BHLHB33, HRT3
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
This gene encodes a member of the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcription factors. The sequence of the encoded protein contains a conserved bHLH and orange domain, but its YRPW motif has diverged from other HESR family members. It is thought to be an effector of Notch signaling and a regulator of cell fate decisions. Alternatively spliced transcript variants have been found, but their biological validity has not been determined. [provided by RefSeq].
Background References:
  • Kokubo, H., et al. Trends Cardiovasc. Med. 15(5):190-194(2005)
  • Colland, F., et al. Genome Res. 14(7):1324-1332(2004)
  • Iso, T., et al. J. Cell. Physiol. 194(3):237-255(2003)
  • Nakagawa, O., et al. Proc. Natl. Acad. Sci. U.S.A. 97(25):13655-13660(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This HEYL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 26-54 amino acids from the N-terminal region of human HEYL.
NCBI Gene ID #:
26508
NCBI Official Name:
Hairy/enhancer-of-split related with YRPW motif-like protein
NCBI Official Symbol:
HEYL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
35 kDa
Protein Accession #:
Q9NQ87
Protein GI Number:
146286205
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NQ87
User NOte:
Optimal dilutions for each application to be determined by the researcher.