EWSR1 Antibody

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ProSci
Product Code: PSI-63-659
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-659-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Fluorescent confocal image of U251 cell stained with EWSR1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.
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Western blot analysis of EWSR1 Antibody in A2058 cell line lysates (35ug/lane)
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Fluorescent confocal image of U251 cell stained with EWSR1 Antibody .U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EWSR1 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).EWSR1 immunoreactivity is localized to Nucleus significantly.
Western blot analysis of EWSR1 Antibody in A2058 cell line lysates (35ug/lane)

Documents

Further Information

Additional Names:
RNA-binding protein EWS, EWS oncogene, Ewing sarcoma breakpoint region 1 protein, EWSR1, EWS
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
EWSR1 is a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain.
Background References:
  • Bhagirath,T., et.al., Genes Chromosomes Cancer 13 (2), 126-132 (1995)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EWSR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 628-656 amino acids from the C-terminal region of human EWSR1.
NCBI Gene ID #:
2130
NCBI Official Name:
RNA-binding protein EWS
NCBI Official Symbol:
EWSR1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q01844
Protein GI Number:
544261
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q01844
User NOte:
Optimal dilutions for each application to be determined by the researcher.