PRP4 Antibody

ProSci
Product Code: PSI-62-982
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-982-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate
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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Western blot analysis of anti-PRP4 Pab in Jurkat cell lysate
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Serine/threonine-protein kinase PRP4 homolog, PRP4 kinase, PRP4 pre-mRNA-processing factor 4 homolog, PRPF4B, KIAA0536, PRP4, PRP4H, PRP4K
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
Pre-mRNA splicing occurs in two sequential transesterification steps, and PRP4 is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs).
Background References:
  • Dellaire, G., et al., Mol. Cell. Biol. 22(14):5141-5156 (2002).
  • Kojima, T., et al., J. Biol. Chem. 276(34):32247-32256 (2001).
  • Huang, Y., et al., Biochem. Biophys. Res. Commun. 271(2):456-463 (2000).
  • Gross, T., et al., Nucleic Acids Res. 25(5):1028-1035 (1997).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This PRP4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 948-978 amino acids from the C-terminal region of human PRP4.
NCBI Gene ID #:
8899
NCBI Official Name:
Serine/threonine-protein kinase PRP4 homolog
NCBI Official Symbol:
PRPF4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
117 kDa
Protein Accession #:
Q13523
Protein GI Number:
317373526
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q13523
User NOte:
Optimal dilutions for each application to be determined by the researcher.