ING4 Antibody

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ProSci
Product Code: PSI-61-026
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-026-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H.pancreas section using ING4 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Western blot analysis of lysate from T47D cell line, using ING4 Antibody at 1:1000.
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Immunohistochemical analysis of paraffin-embedded H.pancreas section using ING4 Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Western blot analysis of lysate from T47D cell line, using ING4 Antibody at 1:1000.

Documents

Further Information

Additional Names:
Inhibitor of growth protein 4, p29ING4, ING4
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
Component of the HBO1 complex which has a histone H4- specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. Through chromatin acetylation it may function in DNA replication. May inhibit tumor progression by modulating the transcriptional output of signaling pathways which regulate cell proliferation. Can suppress brain tumor angiogenesis through transcriptional repression of RELA/NFKB3 target genes when complexed with RELA. May also specifically suppress loss of contact inhibition elicited by activated oncogenes such as MYC. Represses hypoxia inducible factor's (HIF) activity by interacting with HIF prolyl hydroxylase 2 (EGLN1).
Background References:
  • Shiseki M.,et al.Cancer Res. 63:2373-2378(2003).
  • Unoki M.,et al.J. Biol. Chem. 281:34677-34686(2006).
  • Raho G.,et al.Oncogene 26:5247-5257(2007).
  • Mao Y.M.,et al.Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Chicken, Mouse
Immunogen:
This ING4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 162-195 amino acids from the C-terminal region of human ING4.
NCBI Gene ID #:
51147
NCBI Official Name:
Inhibitor of growth protein 4
NCBI Official Symbol:
ING4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q9UNL4
Protein GI Number:
57012981
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UNL4
User NOte:
Optimal dilutions for each application to be determined by the researcher.