RSPO4 Antibody

ProSci
Product Code: PSI-62-016
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-62-016-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Jurkat cell line lysates (15ug/lane).
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RSPO4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in Jurkat cell line lysates (15ug/lane).
RSPO4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
R-spondin-4, Roof plate-specific spondin-4, hRspo4, RSPO4, C20orf182
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
RSPO4 is a member of the R-spondin family of proteins that share a common domain organization consisting of a signal peptide, cysteine-rich/furin-like domain, thrombospondin domain and a C-terminal basic region. The encoded protein may be involved in activation of Wnt/beta-catenin signaling pathways. Mutations in this gene are associated with anonychia congenital. Alternate splicing results in multiple transcript variants.
Background References:
  • Bergmann, C., et al. Am. J. Hum. Genet. 79(6):1105-1109(2006)
  • Blaydon, D.C., et al. Nat. Genet. 38(11):1245-1247(2006)
  • Kim, K.A., et al. Cell Cycle 5(1):23-26(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RSPO4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human RSPO4.
NCBI Gene ID #:
343637
NCBI Official Name:
R-spondin-4
NCBI Official Symbol:
RSPO4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
26 kDa
Protein Accession #:
Q2I0M5
Protein GI Number:
97189858
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q2I0M5
User NOte:
Optimal dilutions for each application to be determined by the researcher.