GPAA1 Antibody

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ProSci
Product Code: PSI-55-369
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-369-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in U251 cell line lysates (35ug/lane).
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GPAA1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.
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Western blot analysis in U251 cell line lysates (35ug/lane).
GPAA1 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.

Documents

Further Information

Additional Names:
Glycosylphosphatidylinositol anchor attachment 1 protein, GPI anchor attachment protein 1, GAA1 protein homolog, hGAA1, GPAA1, GAA1
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
Posttranslational glycosylphosphatidylinositol (GPI) anchor attachment serves as a general mechanism for linking proteins to the cell surface membrane. The protein encoded by this gene presumably functions in GPI anchoring at the GPI transfer step. The mRNA transcript is ubiquitously expressed in both fetal and adult tissues. The anchor attachment protein 1 contains an N-terminal signal sequence, 1 cAMP- and cGMP-dependent protein kinase phosphorylation site, 1 leucine zipper pattern, 2 potential N-glycosylation sites, and 8 putative transmembrane domains.
Background References:
  • Jiang, W.W., et al. Mol. Cancer 6, 74 (2007) :
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Ho, J.C., et al. Int. J. Cancer 119(6):1330-1337(2006)
  • Vainauskas, S., et al. J. Biol. Chem. 280(16):16402-16409(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GPAA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 46-73 amino acids from the N-terminal region of human GPAA1.
NCBI Gene ID #:
8733
NCBI Official Name:
Glycosylphosphatidylinositol anchor attachment 1 protein
NCBI Official Symbol:
GPAA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
O43292
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O43292
User NOte:
Optimal dilutions for each application to be determined by the researcher.