BAHD1 Antibody

ProSci
Product Code: PSI-63-812
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-812-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Fluorescence-activated cell sorting (FACS)
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of BAHD1 Antibody in MDA-MB231 cell line lysates (35ug/lane)
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Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of BAHD1 Antibody in MDA-MB231 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human testis tissue reacted with BAHD1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Further Information

Additional Names:
Bromo adjacent homology domain-containing 1 protein, BAH domain-containing protein 1, BAHD1, KIAA0945
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100

For FACS starting dilution is: 1:10~50
Background:
BAHD1 is a heterochromatin protein that acts as a transcription repressor and has the ability to promote the formation of large heterochromatic domains. It may act by recruiting heterochromatin proteins such as CBX5 (HP1 alpha), HDAC5 and MBD1. It represses IGF2 expression by binding to its CpG-rich P3 promoter and recruiting heterochromatin proteins.
Background References:
  • Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
  • Dephoure N., et.al., Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This BAHD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 750-777 amino acids from the C-terminal region of human BAHD1.
NCBI Gene ID #:
22893
NCBI Official Name:
Bromo adjacent homology domain-containing 1 protein
NCBI Official Symbol:
BAHD1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
85 kDa
Protein Accession #:
Q8TBE0
Protein GI Number:
152040006
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8TBE0
User NOte:
Optimal dilutions for each application to be determined by the researcher.