POLR3C Antibody
| Code | Size | Price |
|---|
| PSI-59-040-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA-directed RNA polymerase III subunit RPC3, RNA polymerase III subunit C3, DNA-directed RNA polymerase III subunit C, RNA polymerase III 62 kDa subunit, RPC62, POLR3C
Application Note:
For WB starting dilution is: 1:1000
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. May direct with other members of the subcomplex RNA Pol III binding to the TFIIIB-DNA complex via the interactions between TFIIIB and POLR3F. May be involved either in the recruitment and stabilization of the subcomplex within RNA polymerase III, or in stimulating catalytic functions of other subunits during initiation. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF-Kappa-B through the RIG-I pathway.
Background References:
- Ablasser, A., et al. Nat. Immunol. 10(10):1065-1072(2009)
- Hu, P., et al. Mol. Cell. Biol. 22(22):8044-8055(2002)
- Kuwana, M., et al. Arthritis Rheum. 46(10):2742-2747(2002)
- Hsieh, Y.J., et al. Mol. Cell. Biol. 19(11):7697-7704(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLR3C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 202-230 amino acids from the Central region of human POLR3C.
NCBI Gene ID #:
10623
NCBI Official Name:
DNA-directed RNA polymerase III subunit RPC3
NCBI Official Symbol:
POLR3C
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
61 kDa
Protein Accession #:
Q9BUI4
Protein GI Number:
60393871
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9BUI4
User NOte:
Optimal dilutions for each application to be determined by the researcher.