ONECUT3 Antibody

ProSci
Product Code: PSI-59-624
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-59-624-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.

Western Blot at 1:1000 dilution + MDA-MB-231 whole cell lysate Lysates/proteins at 20 ug per lane.

Further Information

Additional Names:
One cut domain family member 3, One cut homeobox 3, Transcription factor ONECUT-3, OC-3, ONECUT3
Application Note:
For WB starting dilution is: 1:1000
Background:
ONECUT3 is transcriptional activator. It binds the consensus DNA sequence 5'-DHWATTGAYTWWD-3' on a variety of gene promoters such as those of HNF3B and TTR (By similarity).
Background References:
  • Grimwood, J., et al. Nature 428(6982):529-535(2004)
  • Vanhorenbeeck, V., et al. Biochem. Biophys. Res. Commun. 292(4):848-854(2002)
  • Venter, J.C., et al. Science 291(5507):1304-1351(2001)
  • Jacquemin, P., et al. J. Biol. Chem. 274(5):2665-2671(1999)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This ONECUT3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 393-419 amino acids from the C-terminal region of human ONECUT3.
NCBI Gene ID #:
390874
NCBI Official Name:
One cut domain family member 3
NCBI Official Symbol:
ONECUT3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
O60422
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O60422
User NOte:
Optimal dilutions for each application to be determined by the researcher.