BRF1 Antibody

ProSci
Product Code: PSI-57-998
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-998-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).

Western blot analysis in Hela cell line lysates (35ug/lane).

Further Information

Additional Names:
Transcription factor IIIB 90 kDa subunit, TFIIIB90, hTFIIIB90, B-related factor 1, BRF-1, hBRF, TAF3B2, TATA box-binding protein-associated factor, RNA polymerase III, subunit 2, BRF1, BRF, GTF3B, TAF3B2, TAF3C
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Two alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. Other transcript variants are possible, but their full-length natures have not been completely characterized. [provided by RefSeq].
Background References:
  • Haurie, V., et al. Proc. Natl. Acad. Sci. U.S.A. 107(9):4176-4181(2010)
  • Maitra, S., et al. RNA 14(5):950-959(2008)
  • Leal, J.F., et al. Oncogene 27(14):1961-1970(2008)
  • Yoo, N.K., et al. Cell. Microbiol. 10(3):583-592(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This BRF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 314-342 amino acids from the Central region of human BRF1.
NCBI Gene ID #:
2972
NCBI Official Name:
Transcription factor IIIB 90 kDa subunit
NCBI Official Symbol:
BRF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
74 kDa
Protein Accession #:
Q92994
Protein GI Number:
20455319
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q92994
User NOte:
Optimal dilutions for each application to be determined by the researcher.