UBTF Antibody

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ProSci
Product Code: PSI-56-496
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-56-496-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in K562 cell line lysates (35ug/lane).
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Western blot analysis in K562 cell line lysates (35ug/lane).

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Further Information

Additional Names:
Nucleolar transcription factor 1, Autoantigen NOR-90, Upstream-binding factor 1, UBF-1, UBTF, UBF, UBF1
Application Note:
For WB starting dilution is: 1:1000
Background:
Upstream binding factor (UBF) is a transcription factor required for expression of the 18S, 5.8S, and 28S ribosomal RNAs, along with SL1 (a complex of TBP (MIM 600075) and multiple TBP-associated factors or 'TAFs'). Two UBF polypeptides, of 94 and 97 kD, exist in the human (Bell et al., 1988 [PubMed 3413483]). UBF is a nucleolar phosphoprotein with both DNA binding and transactivation domains. Sequence-specific DNA binding to the core and upstream control elements of the human rRNA promoter is mediated through several HMG boxes (Jantzen et al., 1990 [PubMed 2330041]).
Background References:
  • Hisaoka, M., et al. Mol. Cell. Biol. 30(20):4952-4964(2010)
  • Liu, Z., et al. Proteins 77(2):432-447(2009)
  • Lin, C.I., et al. Biochem. Biophys. Res. Commun. 386(2):396-401(2009)
  • Grob, A., et al. J. Cell. Sci. 122 (PT 4), 489-498 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This UBTF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 631-660 amino acids from the C-terminal region of human UBTF.
NCBI Gene ID #:
7343
NCBI Official Name:
Nucleolar transcription factor 1
NCBI Official Symbol:
UBTF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
89 kDa
Protein Accession #:
P17480
Protein GI Number:
136652
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P17480
User NOte:
Optimal dilutions for each application to be determined by the researcher.