MED30 Antibody

ProSci
Product Code: PSI-60-343
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-343-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in WiDr cell line lysates (35ug/lane).

Western blot analysis in WiDr cell line lysates (35ug/lane).

Further Information

Additional Names:
Mediator of RNA polymerase II transcription subunit 30, Mediator complex subunit 30, TRAP/Mediator complex component TRAP25, Thyroid hormone receptor-associated protein 6, Thyroid hormone receptor-associated protein complex 25 kDa component, Trap25, MED30, THRAP6, TRAP25
Application Note:
For WB starting dilution is: 1:1000
Background:
The multiprotein TRAP/Mediator complex facilitates gene expression through a wide variety of transcriptional activators. MED30 is a component of this complex that appears to be metazoan specific (Baek et al., 2002 [PubMed 11909976]).
Background References:
  • Rienzo, M., et al. Biochim. Biophys. Acta 1799(7):487-495(2010)
  • Sato, S., et al. Mol. Cell 14(5):685-691(2004)
  • Tomomori-Sato, C., et al. J. Biol. Chem. 279(7):5846-5851(2004)
  • Sato, S., et al. J. Biol. Chem. 278(17):15123-15127(2003)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This MED30 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 107-133 amino acids from the Central region of human MED30.
NCBI Gene ID #:
90390
NCBI Official Name:
Mediator of RNA polymerase II transcription subunit 30
NCBI Official Symbol:
MED30
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
Q96HR3
Protein GI Number:
74731968
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96HR3
User NOte:
Optimal dilutions for each application to be determined by the researcher.