POLR1A Antibody
| Code | Size | Price |
|---|
| PSI-60-237-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA-directed RNA polymerase I subunit RPA1, RNA polymerase I subunit A1, A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, RNA polymerase I 194 kDa subunit, RPA194, POLR1A
Application Note:
For WB starting dilution is: 1:1000
Background:
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Largest and catalytic core component of RNA polymerase I which synthesizes ribosomal RNA precursors. Forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol I. A bridging helix emanates from RPA1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol I by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition (By similarity).
Background References:
- Rose, J. Phd, et al. Mol. Med. (2010) In pressPercipalle, P., et al. EMBO Rep. 7(5):525-530(2006) Bouwmeester, T., et al. Nat. Cell Biol. 6(2):97-105(2004) Hirschler-Laszkiewicz, I., et al. J. Biol. Chem. 278(21):18953-18959(2003) Dundr, M., et al. Science 298(5598):1623-1626(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1437-1465 amino acids from the C-terminal region of human POLR1A.
NCBI Gene ID #:
25885
NCBI Official Name:
DNA-directed RNA polymerase I subunit RPA1
NCBI Official Symbol:
POLR1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
195 kDa
Protein Accession #:
O95602
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O95602
User NOte:
Optimal dilutions for each application to be determined by the researcher.