GTF2F2 Antibody

ProSci
Product Code: PSI-60-908
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-908-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in WiDr cell line lysates (35ug/lane).

Western blot analysis in WiDr cell line lysates (35ug/lane).

Further Information

Additional Names:
General transcription factor IIF subunit 2, ATP-dependent helicase GTF2F2, General transcription factor IIF 30 kDa subunit, Transcription initiation factor IIF subunit beta, TFIIF-beta, Transcription initiation factor RAP30, GTF2F2, RAP30
Application Note:
For WB starting dilution is: 1:1000
Background:
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation. This subunit shows ATP-dependent DNA-helicase activity.
Background References:
  • Yang, A., et al. Biochemistry 48(9):1964-1974(2009)
  • Matsuoka, S., et al. Science 316(5828):1160-1166(2007)
  • Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)
  • Le, T.T., et al. J. Biochem. 138(3):215-224(2005)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse, Rat
Immunogen:
This GTF2F2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-172 amino acids from the Central region of human GTF2F2.
NCBI Gene ID #:
2963
NCBI Official Name:
General transcription factor IIF subunit 2
NCBI Official Symbol:
GTF2F2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
28 kDa
Protein Accession #:
P13984
Protein GI Number:
464519
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P13984
User NOte:
Optimal dilutions for each application to be determined by the researcher.