GTF2F1 Antibody

ProSci
Product Code: PSI-59-014
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-59-014-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in T47D cell line lysates (35ug/lane).

Western blot analysis in T47D cell line lysates (35ug/lane).

Further Information

Additional Names:
General transcription factor IIF subunit 1, General transcription factor IIF 74 kDa subunit, Transcription initiation factor IIF subunit alpha, TFIIF-alpha, Transcription initiation factor RAP74, GTF2F1, RAP74
Application Note:
For WB starting dilution is: 1:1000
Background:
TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.
Background References:
  • Yang, A., et al. Biochemistry 48(9):1964-1974(2009)
  • Vega, A., et al. Gynecol. Oncol. 112(1):210-214(2009)
  • Lavery, D.N., et al. Biochemistry 47(11):3352-3359(2008)
  • Cojocaru, M., et al. Biochem. J. 409(1):139-147(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This GTF2F1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-206 amino acids from the Central region of human GTF2F1.
NCBI Gene ID #:
2962
NCBI Official Name:
General transcription factor IIF subunit 1
NCBI Official Symbol:
GTF2F1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
P35269
Protein GI Number:
46397744
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P35269
User NOte:
Optimal dilutions for each application to be determined by the researcher.