NEIL3 Antibody

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ProSci
Product Code: PSI-60-519
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-60-519-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
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Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Western Blot at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Endonuclease 8-like 3, 322-, DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease VIII-like 3, Nei-like protein 3, NEIL3
Application Note:
For WB starting dilution is: 1:1000
Background:
NEIL3 belongs to a class of DNA glycosylases homologous to the bacterial Fpg/Nei family. These glycosylases initiate the first step in base excision repair by cleaving bases damaged by reactive oxygen species and introducing a DNA strand break via the associated lyase reaction (Bandaru et al., 2002 [PubMed 12509226]).
Background References:
  • Krokeide, S.Z., et al. Protein Expr. Purif. 65(2):160-164(2009)
  • Takao, M., et al. Genes Cells 14(2):261-270(2009)
  • Dallosso, A.R., et al. Gut 57(9):1252-1255(2008)
  • Bethke, L., et al. J. Natl. Cancer Inst. 100(4):270-276(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This NEIL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 292-320 amino acids from the Central region of human NEIL3.
NCBI Gene ID #:
55247
NCBI Official Name:
Endonuclease 8-like 3
NCBI Official Symbol:
NEIL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
68 kDa
Protein Accession #:
Q8TAT5
Protein GI Number:
302393810
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8TAT5
User NOte:
Optimal dilutions for each application to be determined by the researcher.