POLK Antibody
| Code | Size | Price |
|---|
| PSI-60-812-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
DNA polymerase kappa, DINB protein, DINP, POLK, DINB1
Application Note:
For WB starting dilution is: 1:1000
Background:
External and internal DNA-damaging agents continually
threaten the integrity of genetic material in cells. Although a
variety of repair mechanisms exist to remove the resulting lesions,
some lesions escape repair and block the replication machinery.
Members of the Y family of DNA polymerases, such as POLK, permit
the continuity of the replication fork by allowing replication
through such DNA lesions. Each Y family polymerase has a unique
DNA-damage bypass and fidelity profile. POLK is specialized for the
extension step of lesion bypass (summary by Lone et al., 2007
[PubMed 17317631]).
Background References:
- Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
- Monsees, G.M., et al. Breast Cancer Res. Treat. (2010) In press :
- Katafuchi, A., et al. Nucleic Acids Res. 38(3):859-867(2010)
- Fukuda, H., et al. J. Biol. Chem. 284(38):25585-25592(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This POLK antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 531-560 amino acids from the Central region of human POLK.
NCBI Gene ID #:
51426
NCBI Official Name:
DNA polymerase kappa
NCBI Official Symbol:
POLK
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
99 kDa
Protein Accession #:
Q9UBT6
Protein GI Number:
59798438
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UBT6
User NOte:
Optimal dilutions for each application to be determined by the researcher.