TFAP2D Antibody

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ProSci
Product Code: PSI-59-530
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-530-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Fluorescent image of A549 cell stained with TFAP2D Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TFAP2D immunoreactivity is localized to Nucleus significantly.
2 / 2
Western blot analysis in HL-60 cell line lysates (35ug/lane).
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Fluorescent image of A549 cell stained with TFAP2D Antibody .A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TFAP2D primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml).TFAP2D immunoreactivity is localized to Nucleus significantly.
Western blot analysis in HL-60 cell line lysates (35ug/lane).

Documents

Further Information

Additional Names:
Transcription factor AP-2-delta, AP2-delta, Activating enhancer-binding protein 2-delta, Transcription factor AP-2-beta-like 1, TFAP2D (HGNC:15581)
Application Note:
For IF starting dilution is: 1:10~50

For WB starting dilution is: 1:1000
Background:
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC (By similarity).
Background References:
  • Kim, J.M., et al. DNA Res. 13(6):275-286(2006)
  • Wenke, A.K., et al. Biochem. Biophys. Res. Commun. 345(1):495-501(2006)
  • Cheng, C., et al. Int. J. Biochem. Cell Biol. 34(1):78-86(2002)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TFAP2D antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 308-336 amino acids from the C-terminal region of human TFAP2D.
NCBI Gene ID #:
83741
NCBI Official Name:
Transcription factor AP-2-delta
NCBI Official Symbol:
TFAP2D
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
Q7Z6R9
Protein GI Number:
74750179
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q7Z6R9
User NOte:
Optimal dilutions for each application to be determined by the researcher.