RNH1 Antibody

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ProSci
Product Code: PSI-56-428
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-428-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunofluorescence (IF)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysate from HT29 cell line,using RNH1 Antibody at 1:1000 at each lane
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Confocal immunofluorescent analysis of RNH1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
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Western blot analysis of lysate from HT29 cell line,using RNH1 Antibody at 1:1000 at each lane
Confocal immunofluorescent analysis of RNH1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Documents

Further Information

Additional Names:
Ribonuclease inhibitor, Placental ribonuclease inhibitor, Placental RNase inhibitor, Ribonuclease/angiogenin inhibitor 1, RAI, RNH1, PRI, RNH
Application Note:
For WB starting dilution is: 1:1000

For IF starting dilution is: 1:10~50
Background:
Placental ribonuclease inhibitor (PRI) is a member of a family of proteinaceous cytoplasmic RNase inhibitors that occur in many tissues and bind to both intracellular and extracellular RNases (summarized by Lee et al., 1988 [PubMed 3219362]). In addition to control of intracellular RNases, the inhibitor may have a role in the regulation of angiogenin (MIM 105850). Ribonuclease inhibitor, of 50,000 Da, binds to ribonucleases and holds them in a latent form. Since neutral and alkaline ribonucleases probably play a critical role in the turnover of RNA in eukaryotic cells, RNH may be essential for control of mRNA turnover; the interaction of eukaryotic cells with ribonuclease may be reversible in vivo.
Background References:
  • Martins-de-Souza, D., et al.Psychiatr Res 44(14):989-991(2010)
  • Martins-de-Souza, D., et al.Neural Transm 116(3):275-289(2009)
  • Turcotte, R.F., et al. Biochem. Biophys. Res. Commun. 377(2):512-514(2008)
  • Johnson, R.J., et al. Biochemistry 46(45):13131-13140(2007)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This RNH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 425-454 amino acids from the C-terminal region of human RNH1.
NCBI Gene ID #:
6050
NCBI Official Name:
Ribonuclease inhibitor
NCBI Official Symbol:
RNH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
50 kDa
Protein Accession #:
P13489
Protein GI Number:
132573
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P13489
User NOte:
Optimal dilutions for each application to be determined by the researcher.