DDX6 Antibody

ProSci
Product Code: PSI-64-175
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-64-175-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
2 / 2
DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Western blot analysis in Hela,MCF-7 cell line lysates (35ug/lane).
DDX6 Antibody IHC analysis in formalin fixed and paraffin embedded breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX6, ATP-dependent RNA helicase p54, DEAD box protein 6, Oncogene RCK, DDX6, HLR2, RCK
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:50~100
Background:
This gene encodes a member of the DEAD box protein family. The protein is an RNA helicase found in P-bodies and stress granules, and functions in translation suppression and mRNA degradation. It is required for microRNA-induced gene silencing.
Background References:
  • Broytman, O., et al. Neurobiol. Aging 30(12):1962-1974(2009)
  • Han, J.W., et al. Nat. Genet. 41(11):1234-1237(2009)
  • Minshall, N., et al. Mol. Biol. Cell 20(9):2464-2472(2009)
  • Tritschler, F., et al. Mol. Cell 33(5):661-668(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Chicken, Mouse, Xenopus
Immunogen:
This DDX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 348-375 amino acids from the Central region of human DDX6.
NCBI Gene ID #:
1656
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX6
NCBI Official Symbol:
DDX6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
54 kDa
Protein Accession #:
P26196
Protein GI Number:
116241327
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P26196
User NOte:
Optimal dilutions for each application to be determined by the researcher.