SARS2 Antibody

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ProSci
Product Code: PSI-63-227
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-63-227-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from U-87 MG, Hela, 293 cell line and mouse skeletal muscle tissue lysate(from left to right), using SARS2 Antibody at 1:1000 at each lane.
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Formalin-fixed and paraffin-embedded human brain tissue reacted with SARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
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Western blot analysis of lysates from U-87 MG, Hela, 293 cell line and mouse skeletal muscle tissue lysate(from left to right), using SARS2 Antibody at 1:1000 at each lane.
Formalin-fixed and paraffin-embedded human brain tissue reacted with SARS2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Documents

Further Information

Additional Names:
Serine--tRNA ligase, mitochondrial, SerRSmt, Seryl-tRNA synthetase, SerRS, Seryl-tRNA(Ser/Sec) synthetase, SARS2, SARSM
Application Note:
For WB starting dilution is: 1:1000

For IHC-P starting dilution is: 1:10~50
Background:
SARS2 catalyzes the attachment of serine to tRNA(Ser). It is also able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).
Background References:
  • Muller,T., Acta Neuropathol. 110 (4), 426-430 (2005)
  • Gibbons,W.J. Jr., Biochem. Biophys. Res. Commun. 317 (3), 774-778 (2004)
  • Yokogawa,T., J. Biol. Chem. 275 (26), 19913-19920 (2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This SARS2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-188 amino acids from the N-terminal region of human SARS2.
NCBI Gene ID #:
54938
NCBI Official Name:
Serine--tRNA ligase, mitochondrial
NCBI Official Symbol:
SARS2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
58 kDa
Protein Accession #:
Q9NP81
Protein GI Number:
23822219
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q9NP81
User NOte:
Optimal dilutions for each application to be determined by the researcher.