TFIP11 Antibody

ProSci
Product Code: PSI-60-873
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-60-873-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Immunohistochemistry- Paraffin Embedded (IHC-P)
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Immunohistochemical analysis of paraffin-embedded H. liver section using TFIP11 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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Western blot analysis in Hela, K562 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the TFIP11 antibody detected the TFIP11 protein (arrow).

Immunohistochemical analysis of paraffin-embedded H. liver section using TFIP11 Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Western blot analysis in Hela, K562 cell line and rat spleen tissue lysates (35ug/lane).This demonstrates the TFIP11 antibody detected the TFIP11 protein (arrow).

Further Information

Additional Names:
Tuftelin-interacting protein 11, Septin and tuftelin-interacting protein 1, STIP-1, TFIP11, STIP
Application Note:
For IHC-P starting dilution is: 1:25

For WB starting dilution is: 1:1000
Background:
TFIP11 is a nuclear speckle-localized protein that may play a role in spliceosome disassembly in Cajal bodies (Stanek et al., 2008 [PubMed 18367544]).
Background References:
  • Saus, E., et al.Psychiatr Res (2010) In press :
  • Tannukit, S., et al. Biochem. Biophys. Res. Commun. 390(3):1044-1050(2009)
  • Gratacos, M., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 150B (6), 808-816 (2009) :
  • Stanek, D., et al. Mol. Biol. Cell 19(6):2534-2543(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This TFIP11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 42-70 amino acids from the N-terminal region of human TFIP11.
NCBI Gene ID #:
24144
NCBI Official Name:
Tuftelin-interacting protein 11
NCBI Official Symbol:
TFIP11
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
97 kDa
Protein Accession #:
Q9UBB9
Protein GI Number:
22096235
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9UBB9
User NOte:
Optimal dilutions for each application to be determined by the researcher.