Ribophorin 2 Antibody

ProSci
Product Code: PSI-61-383
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-61-383-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Immunohistochemistry- Paraffin Embedded (IHC-P)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.

Further Information

Additional Names:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2, Dolichyl-diphosphooligosaccharide--protein glycosyltransferase 63 kDa subunit, RIBIIR, Ribophorin II, RPN-II, Ribophorin-2, RPN2
Application Note:
For IHC-P starting dilution is: 1:50~100
Background:
RNP2 a type I integral membrane protein found only in the rough endoplasmic reticulum. The encoded protein is part of an N-oligosaccharyl transferase complex that links high mannose oligosaccharides to asparagine residues found in the Asn-X-Ser/Thr consensus motif of nascent polypeptide chains. This protein is similar in sequence to the yeast oligosaccharyl transferase subunit SWP1.
Background References:
  • Kelleher, D.J., et al., Mol. Cell 12(1):101-111 (2003). Fu, J., et al., J. Biol. Chem. 275(6):3984-3990 (2000). Loffler, C., et al., Hum. Genet. 87(2):221-222 (1991). Crimaudo, C., et al., EMBO J. 6(1):75-82 (1987). Stoffel, M., et al., Hum. Mol. Genet.(8), 656 (1992).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Pig, Rat
Immunogen:
This Ribophorin 2 (RPN2) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-46 amino acids from the N-terminal region of human Ribophorin 2 (RPN2).
NCBI Gene ID #:
6185
NCBI Official Name:
Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 2
NCBI Official Symbol:
RPN2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
69 kDa
Protein Accession #:
P04844
Protein GI Number:
9297108
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
P04844
User NOte:
Optimal dilutions for each application to be determined by the researcher.