DDX28 Antibody

ProSci
Product Code: PSI-58-493
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-493-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).

Western blot analysis in A549 cell line lysates (35ug/lane).

Further Information

Additional Names:
Probable ATP-dependent RNA helicase DDX28, Mitochondrial DEAD box protein 28, DDX28, MDDX28
Application Note:
For WB starting dilution is: 1:1000
Background:
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of the DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene is intronless. It encodes an RNA-dependent ATPase. The encoded protein is localized in the mitochondria and the nucleus, and can be transported between the mitochondria and the nucleus. [provided by RefSeq].
Background References:
  • Bogenhagen, D.F., et al. J. Biol. Chem. 283(6):3665-3675(2008)
  • Valgardsdottir, R., et al. J. Biol. Chem. 278(23):21146-21154(2003)
  • Valgardsdottir, R., et al. J. Biol. Chem. 276(34):32056-32063(2001)
  • Simpson, J.C., et al. EMBO Rep. 1(3):287-292(2000)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This DDX28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 370-398 amino acids from the C-terminal region of human DDX28.
NCBI Gene ID #:
55794
NCBI Official Name:
Probable ATP-dependent RNA helicase DDX28
NCBI Official Symbol:
DDX28
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
60 kDa
Protein Accession #:
Q9NUL7
Protein GI Number:
296434476
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q9NUL7
User NOte:
Optimal dilutions for each application to be determined by the researcher.