BOLL Antibody

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ProSci
Product Code: PSI-58-408
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-58-408-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.
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Western Blot at 1:1000 dilution Lane 1: human testis lysate Lane 2: Hela whole cell lysate Lysates/proteins at 20 ug per lane.

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Further Information

Additional Names:
Protein boule-like, BOLL, BOULE
Application Note:
For WB starting dilution is: 1:1000
Background:
BOLL belongs to the DAZ gene family required for germ cell development. It encodes an RNA-binding protein which is more similar to Drosophila Boule than to human proteins encoded by genes DAZ (deleted in azoospermia) or DAZL (deleted in azoospermia-like). Loss of this gene function results in the absence of sperm in semen (azoospermia). Histological studies demonstrated that the primary defect is at the meiotic G2/M transition.
Background References:
  • Chen, P., et al. J. Androl. 31(2):163-168(2010)
  • Kee, K., et al. Nature 462(7270):222-225(2009)
  • Lin, Y.M., et al. J. Clin. Endocrinol. Metab. 94(7):2650-2657(2009)
  • Bilguvar, K., et al. Nat. Genet. 40(12):1472-1477(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This BOLL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 23-51 amino acids from the N-terminal region of human BOLL.
NCBI Gene ID #:
66037
NCBI Official Name:
Protein boule-like
NCBI Official Symbol:
BOLL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
31 kDa
Protein Accession #:
Q8N9W6
Protein GI Number:
44887719
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q8N9W6
User NOte:
Optimal dilutions for each application to be determined by the researcher.