Mouse Rnasen Antibody
| Code | Size | Price |
|---|
| PSI-60-211-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Ribonuclease 3, Protein Drosha, Ribonuclease III, RNase III, Drosha, Etohi2, Rn3, Rnasen
Application Note:
For WB starting dilution is: 1:1000
Background:
Ribonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, RNASEN/DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies (By similarity).
Background References:
- Chong, M.M., et al. Genes Dev. 24(17):1951-1960(2010)
- Yang, J.S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(34):15163-15168(2010)
- Michon, F., et al. Dev. Biol. 340(2):355-368(2010)
- Wu, H., et al. PLoS ONE(10), E7566 (2009) :
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This Mouse Rnasen antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 737-764 amino acids from the Central region of mouse Rnasen.
NCBI Gene ID #:
14000
NCBI Official Name:
Ribonuclease 3
NCBI Official Symbol:
Drosha
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
159 kDa
Protein Accession #:
Q5HZJ0
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q5HZJ0
User NOte:
Optimal dilutions for each application to be determined by the researcher.