SNF8 Antibody

ProSci
Product Code: PSI-59-181
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-59-181-400ul400ul£626.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in A549 cell line lysates (35ug/lane).
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Western blot analysis of SNF8 using rabbit polyclonal SNF8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.

Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis of SNF8 using rabbit polyclonal SNF8 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the SNF8 gene.

Further Information

Additional Names:
Vacuolar-sorting protein SNF8, ELL-associated protein of 30 kDa, ESCRT-II complex subunit VPS22, hVps22, SNF8, EAP30
Application Note:
For WB starting dilution is: 1:1000
Background:
SNF8, VPS25 (MIM 610907), and VPS36 (MIM 610903) form ESCRT-II (endosomal sorting complex required for transport II), a complex involved in endocytosis of ubiquitinated membrane proteins. SNF8, VPS25, and VPS36 are also associated in a multiprotein complex with RNA polymerase II elongation factor (ELL; MIM 600284) (Slagsvold et al., 2005 [PubMed 15755741]; Kamura et al., 2001 [PubMed 11278625]).
Background References:
  • Malerod, L., et al. Traffic 8(11):1617-1629(2007)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Langelier, C., et al. J. Virol. 80(19):9465-9480(2006)
  • Bowers, K., et al. J. Biol. Chem. 281(8):5094-5105(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse, Rat
Immunogen:
This SNF8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 37-65 amino acids from the N-terminal region of human SNF8.
NCBI Gene ID #:
11267
NCBI Official Name:
Vacuolar-sorting protein SNF8
NCBI Official Symbol:
SNF8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
29 kDa
Protein Accession #:
Q96H20
Protein GI Number:
73919323
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q96H20
User NOte:
Optimal dilutions for each application to be determined by the researcher.