RENT1 Antibody
| Code | Size | Price |
|---|
| PSI-60-295-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Regulator of nonsense transcripts 1, 364-, ATP-dependent helicase RENT1, Nonsense mRNA reducing factor 1, NORF1, Up-frameshift suppressor 1 homolog, hUpf1, UPF1, KIAA0221, RENT1
Application Note:
For WB starting dilution is: 1:1000
Background:
RENT1 is part of a post-splicing multiprotein complex involved in both mRNA nuclear export and mRNA surveillance. mRNA surveillance detects exported mRNAs with truncated open reading frames and initiates nonsense-mediated mRNA decay (NMD). When translation ends upstream from the last exon-exon junction, this triggers NMD to degrade mRNAs containing premature stop codons. This protein is located only in the cytoplasm. When translation ends, it interacts with the protein that is a functional homolog of yeast Upf2p to trigger mRNA decapping.
Background References:
- Ohnishi, T., et al., Mol. Cell 12(5):1187-1200 (2003).
- Lykke-Andersen, J., Mol. Cell. Biol. 22(23):8114-8121 (2002).
- Carastro, L.M., et al., Nucleic Acids Res. 30(10):2232-2243 (2002).
- Mendell, J.T., et al., Science 298(5592):419-422 (2002).
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This RENT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the N-terminal region of human RENT1.
NCBI Gene ID #:
5976
NCBI Official Name:
Regulator of nonsense transcripts 1
NCBI Official Symbol:
UPF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
124 kDa
Protein Accession #:
Q92900
Protein GI Number:
17380291
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q92900
User NOte:
Optimal dilutions for each application to be determined by the researcher.