POLR2B Antibody

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ProSci
Product Code: PSI-57-949
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-57-949-400ul400ul£626.00
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Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in 293 cell line lysates (35ug/lane).
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Western blot analysis in 293 cell line lysates (35ug/lane).

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Further Information

Additional Names:
DNA-directed RNA polymerase II subunit RPB2, DNA-directed RNA polymerase II 140 kDa polypeptide, DNA-directed RNA polymerase II subunit B, RNA polymerase II subunit 2, RNA polymerase II subunit B2, POLR2B
Application Note:
For WB starting dilution is: 1:1000
Background:
This gene encodes the second largest subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This subunit, in combination with at least two other polymerase subunits, forms a structure within the polymerase that maintains contact in the active site of the enzyme between the DNA template and the newly synthesized RNA. [provided by RefSeq].
Background References:
  • Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
  • Michiels, S., et al. Carcinogenesis 30(5):763-768(2009)
  • Tu, S., et al. Nat. Struct. Mol. Biol. 15(4):419-421(2008)
  • Lee, J.H., et al. Mol. Cell. Biol. 28(2):609-618(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This POLR2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 797-826 amino acids from the Central region of human POLR2B.
NCBI Gene ID #:
5431
NCBI Official Name:
DNA-directed RNA polymerase II subunit RPB2
NCBI Official Symbol:
POLR2B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
134 kDa
Protein Accession #:
P30876
Protein GI Number:
401012
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P30876
User NOte:
Optimal dilutions for each application to be determined by the researcher.