SGOL1 Antibody
| Code | Size | Price |
|---|
| PSI-59-021-400ul | 400ul | £626.00 |
Quantity:
Prices exclude any Taxes / VAT
Overview
Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Application: Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Documents
Further Information
Additional Names:
Shugoshin-like 1, hSgo1, Serologically defined breast cancer antigen NY-BR-85, SGOL1, SGO1
Application Note:
For WB starting dilution is: 1:1000
Background:
SGOL1 plays a central role in chromosome cohesion during mitosis by preventing premature dissociation of cohesin complex from centromeres after prophase, when most of cohesin complex dissociates from chromosomes arms. May act by preventing phosphorylation of the STAG2 subunit of cohesin complex at the centromere, ensuring cohesin persistence at centromere until cohesin cleavage by ESPL1/separase at anaphase. Essential for proper chromosome segregation during mitosis and this function requires interaction with PPP2R1A. Its phosphorylated form is necessary for chromosome congression and for the proper attachment of spindle microtubule to the kinetochore. Necessary for kinetochore localization of PLK1 and CENPF. May play a role in the tension sensing mechanism of the spindle-assembly checkpoint by regulating PLK1 kinetochore affinity. Isoform 3 plays a role in maintaining centriole cohesion involved in controlling spindle pole integrity.
Background References:
- Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
- Okamoto, N., et al. Genes Cells 15(5):471-484(2010)
- Xu, Z., et al. Mol. Cell 35(4):426-441(2009)
- Gambe, A.E., et al. FEBS Lett. 583(12):1951-1956(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This SGOL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 339-367 amino acids from the Central region of human SGOL1.
NCBI Gene ID #:
151648
NCBI Official Name:
Shugoshin-like 1
NCBI Official Symbol:
SGOL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
64 kDa
Protein Accession #:
Q5FBB7
Protein GI Number:
74741474
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q5FBB7
User NOte:
Optimal dilutions for each application to be determined by the researcher.