EMG1 Antibody

ProSci
Product Code: PSI-55-653
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-55-653-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis in Hela cell line lysates (35ug/lane).
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Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in Hela cell line lysates (35ug/lane).
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Ribosomal RNA small subunit methyltransferase NEP1, 211-, 18S rRNA (pseudouridine(1248)-N1)-methyltransferase, 18S rRNA Psi1248 methyltransferase, Nucleolar protein EMG1 homolog, Protein C2f, Ribosome biogenesis protein NEP1, EMG1 {ECO:0000303|PubMed:19463982}
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes an essential, conserved eukaryotic protein involved in ribosome biogenesis. In yeast, the related protein is a component of the small subunit processome and is essential for biogenesis of the ribosomal 40S subunit. A mutation in this gene has been associated with Bowen-Conradi syndrome.
Background References:
  • Armistead, J., et al. Am. J. Hum. Genet. 84(6):728-739(2009)
  • Lamesch, P., et al. Genomics 89(3):307-315(2007)
  • Lamont, R.E., et al. Am. J. Med. Genet.132A (2), 136-143 (2005) :
  • Bernstein, K.A., et al. Eukaryotic Cell 3(6):1619-1626(2004)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This EMG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-133 amino acids from the Central region of human EMG1.
NCBI Gene ID #:
10436
NCBI Official Name:
Ribosomal RNA small subunit methyltransferase NEP1 {ECO:0000305|PubMed:20047967}
NCBI Official Symbol:
EMG1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
27 kDa
Protein Accession #:
Q92979
Protein GI Number:
20532172
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q92979
User NOte:
Optimal dilutions for each application to be determined by the researcher.