CENPN Antibody

ProSci
Product Code: PSI-61-708
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-61-708-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CENPN Antibody in MCF-7 cell line lysates (35ug/lane)
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Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CENPN Antibody in MCF-7 cell line lysates (35ug/lane)
Flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Centromere protein N, CENP-N, Interphase centromere complex protein 32, CENPN, C16orf60, ICEN32
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The centromere is a specialized chromatin domain, present throughout the cell cycle, that acts as a platform on which the transient assembly of the kinetochore occurs during mitosis. All active centromeres are characterized by the presence of long arrays of nucleosomes in which CENPA (MIM 117139) replaces histone H3 (see MIM 601128). CENPN is an additional factor required for centromere assembly.
Background References:
  • Carroll, C.W., et al. Nat. Cell Biol. 11(7):896-902(2009)
  • Olsen, J.V., et al. Cell 127(3):635-648(2006)
  • Izuta, H., et al. Genes Cells 11(6):673-684(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CENPN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 311-339 amino acids from the C-terminal region of human CENPN.
NCBI Gene ID #:
55839
NCBI Official Name:
Centromere protein N
NCBI Official Symbol:
CENPN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
40 kDa
Protein Accession #:
Q96H22
Protein GI Number:
308153423
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Swissprot #:
Q96H22
User NOte:
Optimal dilutions for each application to be determined by the researcher.