KEL Antibody

ProSci
Product Code: PSI-58-060
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-58-060-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western blot analysis in K562 cell line lysates (35ug/lane).
2 / 2
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis in K562 cell line lysates (35ug/lane).
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Kell blood group glycoprotein, 3424-, CD238, KEL
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
This gene encodes a type II transmembrane glycoprotein that is the highly polymorphic Kell blood group antigen. The Kell glycoprotein links via a single disulfide bond to the XK membrane protein that carries the Kx antigen. The encoded protein contains sequence and structural similarity to members of the neprilysin (M13) family of zinc endopeptidases.
Background References:
  • Di Cristofaro, J., et al.Mol Diagn 12(4):453-460(2010)
  • Boturao-Neto, E., et al. Transfusion 50(3):735-737(2010)
  • Yang, Y., et al. Transfus Med 19(5):235-244(2009)
  • Kormoczi, G.F., et al. Transfusion 49(4):733-739(2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This KEL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-243 amino acids from the Central region of human KEL.
NCBI Gene ID #:
3792
NCBI Official Name:
Kell blood group glycoprotein
NCBI Official Symbol:
KEL
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
83 kDa
Protein Accession #:
P23276
Protein GI Number:
1346376
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
P23276
User NOte:
Optimal dilutions for each application to be determined by the researcher.