CDC5L Antibody

ProSci
Product Code: PSI-63-716
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-63-716-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of CDC5L Antibody in MCF-7 cell line lysates (35ug/lane)
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Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of CDC5L Antibody in MCF-7 cell line lysates (35ug/lane)
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Cell division cycle 5-like protein, Cdc5-like protein, Pombe cdc5-related protein, CDC5L, KIAA0432, PCDC5RP
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
The protein is a significant similarity with Schizosaccharomyces pombe cdc5 gene product, which is a cell cycle regulator important for G2/M transition. This protein has been demonstrated to act as a positive regulator of cell cycle G2/M progression. It was also found to be an essential component of a non-snRNA spliceosome, which contains at least five additional protein factors and is required for the second catalytic step of pre-mRNA splicing.
Background References:
  • Grillari,J., et.al., J. Biol. Chem. 284 (42), 29193-29204 (2009) Zhang,N., et.al., EMBO Rep. 10 (9), 1029-1035 (2009)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
This CDC5L antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 187-213 amino acids from the Central region of human CDC5L.
NCBI Gene ID #:
988
NCBI Official Name:
Cell division cycle 5-like protein
NCBI Official Symbol:
CDC5L
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
92 kDa
Protein Accession #:
Q99459
Protein GI Number:
73619933
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q99459
User NOte:
Optimal dilutions for each application to be determined by the researcher.