TCF21 Antibody

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ProSci
Product Code: PSI-55-924
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-55-924-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

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Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody at 1:1000 at each lane.
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Western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).
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Western blot analysis of lysates from Hela cell line mouse spleen tissue lysate(from left to right), using TCF21 Antibody at 1:1000 at each lane.
Western blot analysis in 293 cell line and human placenta tissue lysates (35ug/lane).

Documents

Further Information

Additional Names:
Transcription factor 21, TCF-21, Capsulin, Class A basic helix-loop-helix protein 23, bHLHa23, Epicardin, Podocyte-expressed 1, Pod-1, TCF21, BHLHA23, POD1
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
TCF21 encodes a transcription factor of the basic helix-loop-helix family. The TCF21 product is mesoderm specific, and expressed in embryonic epicardium, mesenchyme-derived tissues of lung, gut, gonad, and both mesenchymal and glomerular epithelial cells in the kidney. Two transcript variants encoding the same protein have been found for this gene.
Background References:
  • Jugessur, A., et al. PLoS ONE(7), E11493 (2010) :
  • Vieira, A.R., et al. Genet. Med. 10(9):668-674(2008)
  • Melzer, D., et al. PLoS Genet.(5), E1000072 (2008) :
  • Smith, L.T., et al. Proc. Natl. Acad. Sci. U.S.A. 103(4):982-987(2006)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Bovine
Immunogen:
This TCF21 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 144-173 amino acids from the C-terminal region of human TCF21.
NCBI Gene ID #:
6943
NCBI Official Name:
Transcription factor 21
NCBI Official Symbol:
TCF21
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
20 kDa
Protein Accession #:
O43680
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
O43680
User NOte:
Optimal dilutions for each application to be determined by the researcher.