TAF4B Antibody

ProSci
Product Code: PSI-56-253
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-56-253-400ul400ul£626.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: Rabbit Ig
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Target Species: Human
Applications:
  • Flow Cytometry
  • Western Blot (WB)
Storage:
Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Images

1 / 2
Western Blot at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
2 / 2
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western Blot at 1:1000 dilution + Ramos whole cell lysate Lysates/proteins at 20 ug per lane.
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Further Information

Additional Names:
Transcription initiation factor TFIID subunit 4B, Transcription initiation factor TFIID 105 kDa subunit, TAF(II)105, TAFII-105, TAFII105, TAF4B, TAF2C2, TAFII105
Application Note:
For WB starting dilution is: 1:1000

For FACS starting dilution is: 1:10~50
Background:
TATA-binding protein associated factors (TAFs) participate, with TATA binding protein (TBP; MIM 600075), in the formation of the TFIID protein complex (see MIM 313650), which is involved in the initiation of gene transcription by RNA polymerase II (see MIM 180660).
Background References:
  • Kalogeropoulou, M., et al. Mol. Cancer Res. 8(4):554-568(2010)
  • Gazit, K., et al. J. Biol. Chem. 284(39):26286-26296(2009)
  • Knauff, E.A., et al. Hum. Reprod. 24(9):2372-2378(2009)
  • Teye, K., et al. Int. J. Oncol. 33(6):1271-1280(2008)
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This TAF4B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 647-675 amino acids from the C-terminal region of human TAF4B.
NCBI Gene ID #:
6875
NCBI Official Name:
Transcription initiation factor TFIID subunit 4B
NCBI Official Symbol:
TAF4B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
91 kDa
Protein Accession #:
Q92750
Protein GI Number:
119370533
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Swissprot #:
Q92750
User NOte:
Optimal dilutions for each application to be determined by the researcher.